With the continuous upgrading of the sports industry structure and the increasing diversification of talent demand, the traditional practical teaching system for physical education majors faces severe challenges in cultivating interdisciplinary talents in the new era. This paper focuses on the practical teaching system of physical education majors in private colleges and universities, guided by the concept of innovation and entrepreneurship, and explores the construction of a "professional competence + innovative thinking + entrepreneurial practice" three-dimensional integrated practical teaching model. Through literature research, field surveys, and case analysis, this paper systematically sorts out the practical dilemmas of "superficial integration, poor connection, and insignificant effects" of innovation and entrepreneurship education in the current practical teaching of physical education majors. It proposes a dual-track integration path with "modularization + project-based" as the curriculum organization method and "on-campus incubation + off-campus linkage" as the platform support. The research shows that this model can effectively improve students' job adaptability, innovative literacy, and entrepreneurial ability, realize the role transformation from "skill executor" to "industry problem solver", and provide a replicable and promotable practical paradigm for the talent cultivation of physical education majors in private colleges and universities.